Mechanisms of action and effectiveness of ozone against viruses

Scritto il 18/07/2022

While the Omicron (latest variant of Covid) continues to spread and authorities are concerned about how to deal with a probable new viral wave in the autumn, studies are being published that demonstrate the effectiveness of ozone in inactivating viruses, with particular reference to SARS-CoV-2.


A very interesting paper was published on April 21 in the journal “Frontiers”, with the title: “Mechanisms of Action of Ozone Therapy in Emerging Viral Diseases: Immunomodulatory Effects and Therapeutic Advantages with Reference to SARS-CoV-2”. (Ozone Therapy mechanisms of action and beneficial effects in relation to the fight against viruses, in particular SARS-CoV-2):


The journal “Frontiers” covers many academic disciplines and is one of the most read and cited scientific publications in the world. It was founded in 2007 by Henry Markram and Kamila Markram, two neuroscientists from the Swiss Federal Institute of Technology (EPFL) in Lausanne.


The study analyzed several research papers published in the clinical setting in order to summarize the biochemical and biological mechanisms underlying ozone therapy and explaining how ozone and its derivatives increase antioxidant and immunostimulating processes, thus offering a perspective on the developments of this therapy in human viral and microbial diseases, including COVID-19.


The authors note that ozone therapy can be a non-invasive medical procedure with no side effects, applied in the treatment of various pathologies, such as cardiovascular, neurodegenerative, orthopedic, dermatological, gastrointestinal and genitourinary diseases.


It is now well established that the beneficial effects of ozone in biological tissues are linked to its ability to stimulate intracellular signaling and metabolism, to enhance the antioxidant and anti-inflammatory activities of the tissues, and to enhance the immunological response.


The mechanism of killing bacteria by ozone is complex, because ozone attacks numerous cellular constituents until the bacteria lose their shape.


Ozone is able to inactivate lipid-coated viruses through a peroxidation reaction, causing damage to both the lipid envelope and the protein shell.


Thus, the virions, deprived of their capsid, are no longer able to bind to their receptors on the cells and infect them, and therefore are unable to carry out their replication cycle, with a consequent reduction in viral load.


Ozone therapy has been used successfully for the treatment of some patients with chronic Hepatitis B. After one year of ozone treatment, patients tested negative with undetectable viral load levels and restoration of normal hepatic transaminase values, demonstrating liver function recovery.


Five symptomatic patients infected with Ebola, the high-lethal hemorrhagic fever virus, recovered in seven days after performing rectal ozone insufflations.


It has also been observed that ozone is able to kill the SARS-CoV-1 virus in monkey cells in vitro and to benefit patients with bronchopneumonia and neurological complications.


Interestingly, SARS-CoV-2 has 82% sequence homology with SARS-CoV-1. Therefore, some authors, during the first phase of the pandemic, envisaged ozone therapy as a potential treatment for COVID-19.


Lipid-coated viruses are highly susceptible to the action of ozone, and the coronavirus belongs to this type of virus.


The study reports in detail the various clinical works carried out in Italy, Spain and India concerning Covid patients who have been healed thanks to ozone therapy.


In conclusion, the authors underline that “especially in this historical period, in which there is a growing risk of emerging viral infections, it could be useful to design clinical studies on larger cohorts of patients with the aim of finding effective therapeutic concentrations of ozone, but also to understand the role of ozone in the initial phase of the disease and to fully explore all the possible biological effects of this treatment”.


The work in question was written by the Italian researchers: Alessandra Cenci, Iole Macchia, Valentina La Sorsa, Clemente Sbarigia, Valentina Di Donna, Donatella Pietraforte, respectively heads of the Core Facilities, Istituto Superiore di Sanità (ISS), Department of Oncology and Molecular Medicine of the ISS, ISS Research Coordination and Support Service, APS S.p.a., and of the Rehabilitation Unit, IRCCS INRCA, Fermo, Italy.


Antonio Gaspari

Direttore Orbisphera



For a more in-depth knowledge of the healing abilities of ozone, we recommend the book:

Antonio Gaspari, “Ozone: a cure for life”